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Continuous computer controlled production of formate dehydrogenase (FDH) and isolation on a pilot scale

✍ Scribed by Dr.-Ing. Dirk Weuster-Botz; Dipl.-Ing. Holger Paschold; Dipl.-Ing. Bernd Striegel; Dipl.-Ing. Holger Gieren; Prof. Dr. Maria-Regina Kula; Dr. Christian Wandrey

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 651 KB
Volume: 17
Category: Article
ISSN: 0930-7516
DOI: 10.1002/ceat.270170211

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The expression of the recombinant wild-type NAD + -and mutant NADP + -dependent formate dehydrogenases (EC 1.2.1.2., FDH) from the methanol-utilizing bacterium Pseudomonas sp. 101 in Escherichia coli cells has been improved to produce active and soluble enzyme up to the level of 50% of total soluble