Scale-up of hydrophobic interaction chromatography for purification of antitumor antibiotic SN-07

✍ Scribed by S. Ishida; J. Saeki; E. Kumazawa; G. Kawanishi; E. Sada; S. Katoh

• Book ID: 104744179
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 381 KB
• Volume: 4
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00369395

✦ Synopsis

Scale-up of hydrophobic interaction chromatography for purification of SN-07 was studied. The height equivalent to a theoretical plate of the adsorbent gel (Sepaheads FP-PH 12) was kept constant for various superficial liquid velocities and column diameters. The efficiency of purification was also constant for the Sepabeads FP-PH 12 columns ranging from 16 mm to 113 mm in inner diameter. Effects of the ligand concentration on the adsorption capacity were also studied, and the adsorption capacity increased with an increase in the ligand concentration. These effects resulted in decreasing the required amount of salt to purify SN-07.