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Scalable recovery of plasmid DNA based on aqueous two-phase separation

✍ Scribed by Andreas Frerix; Markus Müller; Maria-Regina Kula; Jürgen Hubbuch


Book ID
111718042
Publisher
Portland Press
Year
2005
Tongue
English
Weight
466 KB
Volume
42
Category
Article
ISSN
0885-4513

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## Abstract In the current study we developed a process for the capture of pDNA exploiting the ability of aqueous two‐phase systems to differentiate between different forms of DNA. In these systems scpDNA exhibits a near quantitative partitioning in the salt‐rich bottom phase. The successive recove