Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HBsAg)
β Scribed by Hidenari Adachi; Toshiaki Fukuda; Shuji Funahashi; Tomohiro Kurahori; Eiji Ishikawa
- Book ID
- 115108028
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 323 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0042-9007
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π SIMILAR VOLUMES
A cherniluminescent assay for hepatitis-B surface antigen is described which used an isoluminol derivative as the label. The assay is precise intra-assay CV, 1.96-2.45%; inter-assay CV, 5 2 6 8 . 1 1 % and has a lower detection limit for hepatitis-B surface antigen of 1.3 UA.
One hundred sixty-three persons immunised against hepatitis B with a vaccine containing HBsAg either of adw or ayw subtype were examined for antibodies against the a, d, and y determinants of HBsAg. Sera were tested for antibodies against HBsAg adw and HBsAg ayw separately by a solid-phase radioimmu
Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 poo