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Comparative studies of the immunogenic activity of hepatitis B surface antigen (HBsAg) and hbsag polypeptides

โœ Scribed by Yanuario Sanchez; Irina Ionescu-Matiu; Joseph L. Melnick; Dr. Gordon R. Dreesman

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
805 KB
Volume
11
Category
Article
ISSN
0146-6615

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โœฆ Synopsis

Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenicity in mice of each preparation, administered either in saline suspension or adsorbed to aluminum gel, was compared. The SDS-denatured polypeptides were less immunogenic than intact HBsAg particles, whereas the micelles were more immunogenic. High anti-HBs titcrs were observed in mice immunized with micelle preparations in either saline suspension or adsorbed to aluminum gel for as long as 200 days after a booster inoculation, administered 26 days after the primary dose.

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