𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Sample morphology effects in matrix-assisted laser desorption/ionization mass spectrometry of proteins

✍ Scribed by A. Westman; T. Huth-Fehre; P. Demirev; B. U. R. Sundqvist

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
671 KB
Volume
30
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Millimetre‐sized crystals of 2,5‐dihydroxybenzoic acid and 3,5‐dimethoxy‐4‐hydroxycinnamic acid (sinapinic acid) were grown from a cytochrome c/matrix solution. Effects of sample exposure to UV laser irradiation on the matrix‐assisted laser desorption ionization mass spectra are reported for such single crystals. The exposure was varied by irradiating the same sample spot with different numbers of UV‐laser pulses. The ion yield and mass resolution were monitored as a function of the sample exposure. Further, the minimum deposited laser energy needed for protein ion production (the threshold energy) for single crystals was determined for incidence angles between 35° and 60° with respect to the target surface normal. The results were compared with those obtained for polycrystalline samples containing micrometre‐sized crystals.

📜 SIMILAR VOLUMES

Determination of protein-protein interac
Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry
✍ Farmer, Terry B.; Caprioli, Richard M. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB 👁 2 views

A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, mo

Chemical reactivity in matrix-assisted l
Chemical reactivity in matrix-assisted laser desorption/ionization mass spectrometry
✍ C. Enjalbal; B. Sauvagnat; F. Lamaty; R. Lazaro; J. Martinez; P. Mouchet; F. Rou 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 2 views

During the control of a multistep organic synthesis on a soluble polymer (PEG) by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a chemical reactivity was encountered when the matrix was acidic, for the samples where the amino moiety of the anchored compounds was protected as

Matrix-assisted Laser Desorption/Ionizat
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in Milk Science
✍ Silvia Catinella; Pietro Traldi; Costante Pinelli; Evaristo Dallaturca; Renzo Ma 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 798 KB

The protein content of several cow milks was investigated by matrix-assisted laser desorptionhonization (MALDI) mass spectrometry. In particular, milk from four dinerent breeds of cow (Holstein Frisons, Brown Swiss, Jersey and Reggiana) at the same lactation stage and under the same feeding system w

Decomposition of protein nitrosothiolsin
Decomposition of protein nitrosothiolsin matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry
✍ Rina Kaneko; Yoshinao Wada 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 217 KB 👁 1 views

## Abstract The __S__‐nitrosylation of proteins is involved in the trafficking of nitric oxide (NO) in intra‐ and extracellular milieus. To establish a mass spectrometric method for identifying this post‐translational modification of proteins, a synthetic peptide and transthyretin were __S__‐nitros

Analysis of wheat gluten proteins by mat
Analysis of wheat gluten proteins by matrix-assisted laser desorption/ionization mass spectrometry
✍ Dworschak, Ragnar G.; Ens, Werner; Standing, Kenneth G.; Preston, Ken R.; Marchy 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 237 KB 👁 2 views

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) was used to analyze the protein composition in several common and durum wheat varieties. Mass spectra were obtained directly from crude and partially puriÐed wheat gliadin and reduced glutenin subunit fractions. Mass spectra of