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Saccharomyces cerevisiae DNA-dependent RNA polymerase III: a zinc metalloenzyme

โœ Scribed by Wandzilak, Theodore M.; Benson, Robert W.

Book ID
127026237
Publisher
American Chemical Society
Year
1978
Tongue
English
Weight
769 KB
Volume
17
Category
Article
ISSN
0006-2960

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โœ Grant A. Bitter ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a