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A Specific DNA-Dependent DNA Polymerase Is Associated with Saccharomyces cerevisiae Telomerase

✍ Scribed by A.V. Petrov; P.V. Dmitriev; K.A. Sokolov; O.A. Dontsova

Book ID
110321512
Publisher
Springer
Year
2001
Tongue
English
Weight
431 KB
Volume
66
Category
Article
ISSN
0006-2979

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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a