Rubratoxins
โ Scribed by Moss, M. O.; Robinson, F. V.; Wood, A. B.
- Book ID
- 126302534
- Publisher
- The Royal Society of Chemistry
- Year
- 1971
- Tongue
- English
- Weight
- 771 KB
- Category
- Article
- ISSN
- 0022-4952
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In order to check the hypothesis that rubratoxin B (2), a C,,-metabolite, is formed biogenetically by head-to-tail coupling of two identical C13-precursors derived from decanoic acid and oxaloacetic acid, two labelled forms of the postulat- ## ed C,,-intermediate 2-((E)-l'-o~tenyl)-3-['~C]methyl-an
Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratox
[14C]rubratoxin B was produced by culturing Penicillium rubrum Stoll for 13 days at 22 degrees C in medium containing [14C]glucose. The most efficient incorporation of glucose into rubratoxin occurred when Raulin-Thom medium enriched with 2.5% malt extract was supplemented with 2.5% added glucose. T