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Routine manufacture of recombinant proteins using expanded bed adsorption chromatography

✍ Scribed by Scot R. Shepard; Gregory A. Boyd; Jeffrey L. Schrimsher


Book ID
110309084
Publisher
Springer
Year
2001
Tongue
English
Weight
324 KB
Volume
10
Category
Article
ISSN
1573-8272

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On-column refolding of recombinant human
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A refolding strategy was described for oncolumn refolding of recombinant human interferong (rhIFN-g) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-g protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded