The lambda dv plasmid genome is composed of two regions: (1) the autorepressor region which consists of promoter-operator (pRoR) and autorepressor (tof) and (2) the origin region which consists of the origin of replication (ori) and two initiator genes (O and P) (Matsubara 1976). Replication of this
Role of the tof gene in the production and perpetuation of the λdv plasmid
✍ Scribed by Matsubara, Kenichi ;Takeda, Yoshinori
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 393 KB
- Volume
- 142
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
A series of lambda derivatives carrying tof mutations were tested for their ability to give rise to plasmid lambda dv. Phages carrying tof mutations that distorted expression of the pRoR-tof-OP operon, were unable to produce lambda dv. Phages carrying an altered tof gene, having only a moderate effect on the same operon, produced unstable lambdadv's. On the other hand, those tof mutants were only the expression of the pLoL-N-exo operon, but not that of the pRoR-tof-OP operon was affected, produced stable lambdadv's.
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