Role of the Ly 1 antigen in interleukin 1-induced thymocyte activation

✍ Scribed by Lennart Lögaberg; Ethan M. Shevach

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 751 KB
• Volume: 15
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830151009

✦ Synopsis

Interleukin 1 (IL I), but not IL 2-induced thymocyte proliferation was augmented by monoclonal antibodies (mAb) against the Ly 1 antigen. The anti-Ly 1 mAb could also co-stimulate thymocytes with phytohemagglutinin (PHA) alone, mimicking the effect of IL1. Moreover, overnight culture with PHA of thymocytes, but not of spleen T lymphocytes led to selectively enhanced expression of the L y l antigen on the cell surface. Similar data were generated at the clonal level. Thus, a thymocyte hybridoma (22A6) could be stimulated to release IL2 in response to PHA plus IL 1, but also to PHA plus anti-Ly 1 mAb. In addition, 22A6 exhibited enhanced cell surface expression of the L y l antigen after overnight culture with PHA. These data suggest a critical role for the Ly 1 antigen in thymocyte proliferation, perhaps by serving as an IL 1-receptor.