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Role of the leader sequence in tobacco pectin methylesterase secretion

✍ Scribed by Yuri L. Dorokhov; Eugene V. Skurat; Olga Yu. Frolova; Tatjana V. Gasanova; Peter A. Ivanov; Nikolay V. Ravin; Konstantin G. Skryabin; Kristiina M. Mäkinen; Viktor I. Klimyuk; Yuri Yu. Gleba; Joseph G. Atabekov


Book ID
116466676
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
402 KB
Volume
580
Category
Article
ISSN
0014-5793

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When the secreted bacterial protein ChiA is expressed in transgenic tobacco, a fraction of the protein is glycosylated and secreted from the plant cells; however most of the protein remains inside the cells. We tested whether the efficiency of secretion could be improved by replacing the bacterial s