During the process of degradation of the cell wall of the yeast form of Pullularia pullulans by the lytic system of micromonospora chalcea samples were withdrawn at different times and observed under phase contrast and electron microscope. The progressive lysis of the walls reveals a fibrillar compo
Role of the cell wall in the ability of tobacco protoplasts to form callus
β Scribed by L. Schilde-Rentschler
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 1002 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
Addition of cellulose to the culture medium inhibited cell wall regeneration and nuclear and cell division in tobacco ( N i c o t i a n a tabacum L.) mesophyll protoplasts. When the activity of cellulase was inhibited by cellobiose, the inhibition of nuclear and cell division was overcome. Cell w~tll formation thus appears to be a prerequisite for nuclear and cell division in tobacco protoplasts.
K e y words: Cell division -Cell wall regeneration -Cellulose synthesis -N i c o t i a n a -Protoplast culture.
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A polyclonal antibody raised against cellulase (EC 3.2.1.4.) from callus of Nicotiana tabacum L. cv. Petit Havana SR1 reduced cellulase activity and induced shoot formation in tobacco callus in the presence of callus maintaining concentrations of auxin and cytokinin. Shoot induction as well as reduc
The biochemistry of cell-wall regeneration in protoplasts obtained from Vinca rosea L. (Catharanthus roseus (L.) G. Don) cells grown in suspension culture by isolating the regenerated wall and the extracellular polysaccharides of protoplasts cultured for various periods, and investigating their comp