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Composition of the cell wall formed by protoplasts isolated from cell suspension cultures ofVinca rosea

✍ Scribed by Yuichi Takeuchi; Atsushi Komamine

Publisher: Springer-Verlag
Year: 1978
Tongue: English
Weight: 491 KB
Volume: 140
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00390252

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✦ Synopsis

The biochemistry of cell-wall regeneration in protoplasts obtained from Vinca rosea L. (Catharanthus roseus (L.) G. Don) cells grown in suspension culture by isolating the regenerated wall and the extracellular polysaccharides of protoplasts cultured for various periods, and investigating their composition. Gas-liquid chromatography and tracer studies with D-[U-(14)C]glucose showed that the sugar composition of the extracellular polysaccharides was similar to that of the original cell culture, consisting mainly of polyuronide and 3,6-linked arabinogalactan. the regenerated cell wall was composed of non-cellulosic glucans having 1,3- and 1,4-linkages, while its content in pectic and hemicellulosic components was very low.

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