Characteristic changes in the patterns of protein phosphorylation occur during meiotic maturation of mouse oocytes from the time subsequent to germinal vesicle breakdown, through metaphase 11, and following fertilization. These changes occur during both in vitro or in vivo maturation or fertilizatio
Role of Protein Phosphorylation in Meiotic Maturation of Mouse Oocytes in Vitro
β Scribed by RICHARD M. SCHULTZ
- Book ID
- 119860893
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 964 KB
- Volume
- 541
- Category
- Article
- ISSN
- 0890-6564
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Cumulus-enclosed sheep oocytes were cultured in gonadotrophin-containing medium for up to 9 hr and were then incubated for 3 hr in the presence of [32P]phosphate. The incorporation of 32P into TCA-insoluble material was measured, and oocyte proteins were separated by one-and twodimensional gel elect