Role of mitochondria in ethanol tolerance ofSaccharomyces cerevisiae

✍ Scribed by Andrés Aguilera; Tahía Benítez

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 429 KB
• Volume: 142
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00491909

✦ Synopsis

The presence of active mitochondria and oxidative metabolism is shown to be essential to maintain low inhibition levels by ethanol of the growth rate (#), fermentation rate (v) or respiration rate (Q) of Saccharomyces cerevisiae wild type strain $288C. Cells which have respiratory metabolism show Ki (ethanol inhibition constant) values for/~, v and Q, higher (Ki > 1 M) than those of "petite" mutants or "grande" strains grown in anaerobiosis (Ki = 0.7 M). In addition, the relationship between p or v and ethanol concentration is linear in cells with respiratory metabolism and exponential in cells lacking respiration. When functional mitochondria are transferred to "petite" mutants, the resulting strain shows Ki values similar to those of the "grande" strain and the inhibition of # and v by increasing ethanol concentrations becomes linear.