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Role of DNA–DNA interactions on the structure and thermodynamics of bacteriophages Lambda and P4

✍ Scribed by Anton S. Petrov; Stephen C. Harvey

Book ID
116697140
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
998 KB
Volume
174
Category
Article
ISSN
1047-8477

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A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/