Role of Calcium Ions in the Thermostability of Thermolysin and Bacillus subtilis var. amylosacchariticus Neutral Protease
โ Scribed by Masahiro TAJIMA; Itaru URABE; Katsuhide YUTANI; Hirosuke OKADA
- Book ID
- 115115313
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 428 KB
- Volume
- 64
- Category
- Article
- ISSN
- 1432-1327
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## Abstract Thermolysin and other secreted broadโspecificity proteases, such as subtilisin or alphaโlytic protease, are produced as preโproโproteins that stay at least partially unfolded while in the cytosol. After secretion, the proโproteases fold to their active conformations in a process that in
## Abstract Engineered extremely thermostable variants of the thermolysinโlike protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56โ69 (Boilysin, BLN) have been pr