Role of calcium in apoptosis of HL-60 cells induced by harringtonine
โ Scribed by Min Fang; Hongqing Zhang; Shaobai Xue
- Publisher
- Science in China Press (SCP)
- Year
- 1998
- Tongue
- English
- Weight
- 790 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1674-7305
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Treatment of HL-60 cells with micromolar concentrations of N 6 -benzyladenosine (N 6 -benzylaminopurine riboside [BAPR]) led to the occurrence of apoptosis in a concentration-dependent manner. Incubation period as short as 2 h in the presence of BAPR was sufficient for triggering irreversible change
Cells of the human promyelocytic HL-60 line, when treated with a variety of antitumor agents in the presence of the protein synthesis inhibitor cycloheximide (CHX), or with CHX alone, rapidly undergo apoptosis ("active cell death"). It is presumed, therefore, that such cells are "primed" to apoptosi