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Role of Amino-acids in the Germination of Bacterial Spores by Subtilisin

โœ Scribed by GOULD, G. W.; KING, W. L.


Book ID
109652240
Publisher
Nature Publishing Group
Year
1966
Tongue
English
Weight
236 KB
Volume
211
Category
Article
ISSN
0028-0836

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Determination of dipicolinic acid in bac
โœ Alan D. Warth ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 276 KB

Dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mM HCl for 10 min at 100 degrees C. The suspension was diluted with 5 mM Ca2+, 100 mM Tris, pH 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285