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Robustness of an Immobilized Artificial Membrane High-Performance Liquid Chromatography Method for the Determination of Lipophilicity

✍ Scribed by Ledbetter, Moira R.; Gutsell, Steve; Hodges, Geoff; Madden, Judith C.; Rowe, Philip H.; Cronin, Mark T. D.

Book ID
127264751
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
527 KB
Volume
57
Category
Article
ISSN
0021-9568

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Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic