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Enzyme-based high-performance liquid chromatography stationary phases as metabolic reactors: Immobilization of non-solubilized rat liver microsomes on an immobilized artificial membrane high-performance liquid chromatography support

✍ Scribed by Tanja Alebić-Kolbah; Irving W. Wainer


Book ID
107785055
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
637 KB
Volume
646
Category
Article
ISSN
1873-3778

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Enzyme-based high-performance liquid chr
✍ Wai-Keung Chui; Irving W. Wainer 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 939 KB

Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic