Robotic Manipulation of Reproductive Cells

✍ Scribed by Changsheng Dai, Yu Sun

• Publisher: Springer
• Year: 2024
• Tongue: English
• Leaves: 134
• Category: Library

✦ Synopsis

This is the first book to focus on robotic reproductive cell manipulation. It provides readers with the fundamental principles underpinning robotic manipulation of reproductive cells, including sperm, oocytes, and embryos, state-of-the-art technical advances in actuation, sensing and control for cell manipulation, and emerging automated systems for reproductive cell manipulation. The methods presented in the book are generic and can be translated to manipulating other types of cells, such as cancer cells and cardiomyocytes.
Robotic Manipulation of Reproductive Cells will be an essential reference for graduate students and researchers working on small-scale robotic systems for cell manipulation and characterization, healthcare professionals interested in nanoscale, microscale, milli-scale robotic techniques for clinical cell surgeries and assisted reproduction, and engineers developing small-scale robotic systems for biomedical engineering, biology,and medicine.

✦ Table of Contents

Preface
Contents
Chapter 1: Overview of Robotic Reproductive Cell Manipulation
1.1 Assisted Reproduction Technologies: Past, Present, and Future
1.2 Advances in Robotic Cell Manipulation
1.3 Challenges for Robotic Cell Manipulation in Assisted Reproduction
1.4 Organization of the Book
References
Chapter 2: Automated Sperm Analysis
2.1 Robust Sperm Detection
2.2 Multiple-Sperm Tracking
2.3 Staining-Free Sperm Morphology Measurement
2.4 Clinical Validation of Sperm Analysis System
References
Chapter 3: Robotic Sperm Immobilization
3.1 Rotational Positioning of End-Effector for Sperm Immobilization
3.2 Rotational Stage and Laser-Based Sperm Immobilization to Overcome Orientation Limit
3.3 Piezo-Assisted Sperm Immobilization for Orientation Control and Effective Membrane Ablation
References
Chapter 4: Automated Picoliter-Resolution Sperm Aspiration
4.1 Progress in Cell Aspiration
4.2 Dynamics of Cell Motion During Aspiration
4.3 Adaptive Controller Design
4.4 Results of Automated Sperm Aspiration
References
Chapter 5: Robotic Orientation Control of Linear-Shaped Sperm
5.1 Robust Sperm Tail Segmentation
5.2 Automated Orientation Control of Motile Deformable Sperm
5.3 Robotic Rotation of Immobilized Sperm
References
Chapter 6: Robotic Orientation Control of Spherical Oocytes
6.1 Deep-Learning-Based Polar Body Detection
6.2 Rotational Stage and Visual Servoing for Oocyte Orientation Control
6.3 Robotic Oocyte Rotation with Minimal Deformation
References
Chapter 7: Piezo Drill-Based Minimally Invasive Oocyte Injection
7.1 Piezo Drill Designs to Penetrate Oocytes with Small Deformation
7.2 Automated Oocyte Injection with Piezo Drill
7.3 Experiments on Piezo Drill-Based Cell Injection
References
Chapter 8: Robotic Embryo Characterization and Manipulation
8.1 Embryo 3D Reconstruction by Robotic Rotation and Feature Matching
8.2 Robotic Embryo Biopsy for Genetic Testing
References
Chapter 9: Untethered Robotic Manipulation of Reproductive Cells
9.1 Flow-Based 3D Embryo Rotation
9.2 Magnetic-Based Embryo Manipulation for Mechanical Parameter Measurement
References
Chapter 10: Future Perspectives of Robotic Manipulation of Reproductive Cells
10.1 Emerging Tools for 3D Label-Free Cell Imaging
10.2 Robotic Intracellular Manipulation of Reproductive Cells
10.3 Machine Learning for Robotic Cell Manipulation
References
Index