RNA polymerase activity in liver nuclei of intact and adrenalectomized γ-irradiated mice
✍ Scribed by M. B. Yatvin
- Book ID
- 112647374
- Publisher
- Springer
- Year
- 1970
- Tongue
- English
- Weight
- 119 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1420-682X
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(1) A single injection of methylmercury chloride in the rat (10-50 mg/kg) increased both in vivo and in vitro rates of 14C-leucine incorporation into the protein of the post-mitochondrial supernatant fraction of the liver. In contrast, no stimulation of protein synthesis was observed in the brain of
Nuclei isolated from ischemic liver show a consistent reduction of their RNA synthesis. The reduction persists at high ionic strength and in the presence of heparin, when RNA synthesis in vitro is fully activated and occurs in the presence as well as in the absence of alpha-amanitin. Both the number