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Risk assessment in cervical dysplasia patients by single cell gel electrophoresis assay: a study of DNA damage and repair

✍ Scribed by Anuradha Udumudi; Malini Jaiswal; N Rajeswari; Neena Desai; S Jain; N Balakrishna; K.Visweswara Rao; Y.R Ahuja


Book ID
117767528
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
1015 KB
Volume
412
Category
Article
ISSN
1383-5718

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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph