Rifampicin binding as a probe for subunit interactions in Escherchia coli RNA polymerase
โ Scribed by Peter A. Lowe; Alan D.B. Malcolm
- Book ID
- 115746482
- Publisher
- Elsevier Science
- Year
- 1976
- Weight
- 510 KB
- Volume
- 454
- Category
- Article
- ISSN
- 0005-2787
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๐ SIMILAR VOLUMES
The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid
The transducing phage 2 dsupM814 and the plasmid rpIK A l L rpo 8 plB1830 containing the wild-type rpoB gene have been constructed and the primary structure ยฐf the gene's central fragment l l l has been established. In contrast with the wild-type, the gene g G C of the rpoB255 mutant, whose primary