The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid
A rifampicin resistent rna-polymerase from E. coli altered in the β-subunit
✍ Scribed by D. Rabussay; W. Zillig
- Book ID
- 115901131
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 371 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0014-5793
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We have analyzed some chemical properties of the sigma subunit of RNA polymerase from the sigma mutants: rpoD1 (Gross et al., 1978), rpoD2 (formerly known as alt-1) (Silverstone et al., 1972; Travers et al., 1978), and rpoD800 (Gross et al., 1979). Each of the three mutants is located at about 66 mi
The transducing phage 2 dsupM814 and the plasmid rpIK A l L rpo 8 plB1830 containing the wild-type rpoB gene have been constructed and the primary structure °f the gene's central fragment l l l has been established. In contrast with the wild-type, the gene g G C of the rpoB255 mutant, whose primary