𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Ricin-induced toxicity in the macrophage J744A.1 cells: The role of TNF-α and the modulation effects of TNF-α polyclonal antibody

✍ Scribed by Ezdihar Hassoun; Xin Wang

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
487 KB
Volume
14
Category
Article
ISSN
1095-6670

No coin nor oath required. For personal study only.

✦ Synopsis

Ricin is a natural toxin of the castor beans (Ricinus communus). We studied the time-and concentration-dependent effects of ricin on the release of TNF-␣ and lactate dehydrogenase (LDH), as well as the modulation of the ricin-induced effects by TNF-␣ antibody in the J774A.1 cells. When added at concentrations ranging from 0 to 1000 ng/mL, ricin caused concentration-dependent increases in the release of TNF-␣ after incubation for 12 to 24 hours. Concentration-dependent increases in the leakage of LDH were also observed after incubation of the cells with those concentrations of ricin for 24 to 48 hours. Addition of 5 units/ mL of rabbit anti-mouse TNF-alpha polyclonal antibody (TNF-␣ antibody) 2 hours prior to the addition of ricin resulted in a decrease in the ricin-induced toxicity, indicated by the release of LDH by the cells. However, when added at concentrations higher than 5 units/mL, the antibody resulted in either no effect or an increase in the ricin-induced LDH leakage. These results suggest that secretion of TNF-␣ by the macrophages in response to ricin plays a significant role in the toxicity of ricin and that TNF-␣ antibody can antagonize the effects of ricin in this cell line when added at relatively low concentrations.

📜 SIMILAR VOLUMES

JNK modulates the effect of caspases and
JNK modulates the effect of caspases and NF-κB in the TNF-α-induced down-regulation of Na+/K+ATPase in HepG2 cells
✍ Ari Kassardjian; Sawsan Ibrahim Kreydiyyeh 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB 👁 1 views

## Abstract An inhibition of the Na^+^/K^+^ATPase was previously shown to accompany and potentiate apoptosis in different experimental models. Since TNF‐α is known to be a pro and anti‐apoptotic cytokine, this work was undertaken to study the effect of TNF‐α on the Na^+^/K^+^ATPase in HepG2 cells a

SYNERGISTIC REGULATORY EFFECTS OF TNF&#x
SYNERGISTIC REGULATORY EFFECTS OF TNF α, IL-1 αAND IFN α ON THE GROWTH AND DIFFERENTIATION OF DAUDI LYMPHOMA CELLS
✍ E. SANTAVENERE; R. DI PIETRO; M.A. CENTURIONE; L. DI DOMENICANTONIO; O. TRUBIANI 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 969 KB

## Abstract The regulatory effects of the combined treatment of tumour necrosis factorα (TNFα), interleukin‐1α (IL‐1α) and interferonα(IFNα) on the growth and differentiation of Daudi lymphoma cells were investigated. By means of anti‐BrdU monoclonal antibodies and [^3^H‐thymidine] incorporation a

Effects of inflammatory cytokines IL-1β,
Effects of inflammatory cytokines IL-1β, IL-6, and TNFα on the intracellular localization of retinoid receptors in Schwann cells
✍ Jörg Mey; Kirsten Schrage; Inga Wessels; Ilona Vollpracht-Crijns 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 768 KB

## Abstract It was investigated whether retinoic acid (RA) and the proinflammatory cytokines IL‐1β, IL‐6, and TNFα influence the intracellular distribution of retinoic acid receptors (RAR) and retinoid X receptors (RXR) in Schwann cells. This question arose because nuclear translocation of RARα, RX

Overexpression of HSP25 reduces the leve
Overexpression of HSP25 reduces the level of TNFα-induced oxidative DNA damage biomarker, 8-hydroxy-2′-deoxyguanosine, in L929 cells
✍ Young-Mee Park; Mi-Young Han; Robert V. Blackburn; Yong J. Lee 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB

Previously we and others have demonstrated that oxidative stress involving generation of reactive oxygen species (ROS) is responsible for the cytotoxic action of TNFa. Protective effect of small heat shock proteins (HSP) against diverse oxidative stress conditions has been suggested. Although overex

Role of IκBα and IκBβ in the biphasic nu
Role of IκBα and IκBβ in the biphasic nuclear translocation of NF-κB in TNFα-stimulated astrocytes and in neuroblastoma cells
✍ Iris Kemler; Adriano Fontana 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 113 KB 👁 1 views

In infectious diseases of the central nervous system astrocytes respond to inflammatory cytokines like tumor necrosis factor ␣ (TNF␣) by activation of the transcription factor NF-B, mediated by the proteolysis of its inhibitors IB␣ and IB␤. We studied the kinetics of NF-B induction by TNF␣ in primar