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Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system

โœ Scribed by Kyung-Ho Lee; Yong-Chan Kwon; Sung Joon Yoo; Dong-Myung Kim


Book ID
116829114
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
675 KB
Volume
71
Category
Article
ISSN
1046-5928

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A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from