We describe a mitochondrial suppressor mutation, which restores respiratory competence to the nuclear pet--mutant MK2. This mutant lacks the message of the mitochondrial cob-gene and instead accumulates a partially spliced pre-mRNA which ,is not translated. Complete processing and translation of the
Ribosomal proteins of yeast strains carrying mutations which affect the efficiency of nonsense suppression
β Scribed by Waldron, Clive ;Cox, Brian S.
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 242 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
We have examined the ribosomal proteins of strains of Saccharomyces cerevisiae which differ in the efficiency with which ochre nonsense mutations are suppressed. The strains in which ochre suppression is poor were [psi]- or carried antisuppressor mutations; those in which suppression was highly efficient were [psi]+ or carried allosuppressor mutations. The ribosomal proteins of these strains, as judged by two-dimensional polyacrylamide gel electrophoresis, were indistinguishable from those of wild-type.
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A suppressor mutation of a temperature-sensitive mutant of ribosomal protein L24 (rplX19) was mapped close to the lon gene by genetic analysis and was shown to affect protease LA. The degradation and the synthesis rates of individual ribosomal proteins were determined. Proteins L24, L14, L15 and L27