Ribose 1-phosphate metabolism in Ehrlich ascites tumor cells in vitro
โ Scribed by Jerzy Barankiewicz; J.Frank Henderson
- Book ID
- 115746553
- Publisher
- Elsevier Science
- Year
- 1977
- Weight
- 420 KB
- Volume
- 479
- Category
- Article
- ISSN
- 0005-2787
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The effect of changes in extracellular pH (pH,) and intracellular pH (pHJ on Na+-dependent and Na+-independent inorganic phosphate (Pi) transport in Ehrlich cells was investigated. In the presence of Na+, acutely reducing pH, from 7.30 to 5.50 results first in a transient (-7 min) stimulation of Pi
In an effort to determine whether the Na+-dependent P, transport system of Ehrlich ascites tumor cells exhibits specificity for H,PO, or HPO, :, P, fluxes were determined by measuring "P,-P, self-exchange. Three experimental approaches were employed. First, the effect of pH on steady-state P, transp