Determination of ribose 1-phosphate in ascites tumor cells
โ Scribed by Jerzy Barankiewicz; J.Frank Henderson
- Book ID
- 115766584
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 460 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-2944
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In the course of studies on nucleoside monophosphate metabolism, the need was encountered for a method to determine ribose-l-phosphate. Published assays for ribose-l-phosphate depend either on chromatographic separation of the sugarphosphate, or else on its acid lability which allows it to be determ
The effect of changes in extracellular pH (pH,) and intracellular pH (pHJ on Na+-dependent and Na+-independent inorganic phosphate (Pi) transport in Ehrlich cells was investigated. In the presence of Na+, acutely reducing pH, from 7.30 to 5.50 results first in a transient (-7 min) stimulation of Pi
In an effort to determine whether the Na+-dependent P, transport system of Ehrlich ascites tumor cells exhibits specificity for H,PO, or HPO, :, P, fluxes were determined by measuring "P,-P, self-exchange. Three experimental approaches were employed. First, the effect of pH on steady-state P, transp
The effects of extracellular P, and Na' on cellular Pi concentration and transport were studied. Steady-state P, exchange flux was measured by 3zP uptake in the presence and absence of Na'. Model experiments were also conducted to assess t h e possibility that hydrolysis of organic phosphate esters