Reversible steps in the reaction of aldehydes with bacterial luciferase intermediates
โ Scribed by A.L. Baumstark; T.W. Cline; J.W. Hastings
- Book ID
- 115703926
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 599 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0003-9861
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A t 22ยฐC the bioluminescence decay kinetics in the in vitro reaction catalysed by Vibrio harveyi luciferase in the presence of different aldehydes-nonanal, decanal, tridecanal and tetradecanal did not follow the simple exponential pattern and could be fitted t o a two-exponential process. One more p
Addition of KO 2 in dimethyl sulfoxide (DMSO) to the in vitro bacterial luciferase reaction subsequent to its initiation resulted in a biphasic decay of light emission. The first and more rapid phase is attributed to quenching by DMSO. With DMSO alone the continuing decay is kinetically the same as