Bioluminescence decay kinetics in the reaction of bacterial luciferase with different aldehydes
✍ Scribed by Ismailov, Anvar D. ;Sobolev, Alexander Yu. ;Danilov, Vadim S.
- Publisher
- John Wiley and Sons
- Year
- 1990
- Weight
- 390 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
A t 22°C the bioluminescence decay kinetics in the in vitro reaction catalysed by Vibrio harveyi luciferase in the presence of different aldehydes-nonanal, decanal, tridecanal and tetradecanal did not follow the simple exponential pattern and could be fitted t o a two-exponential process. One more principal distinction from the first-order kinetics is the dependence of the parameters on aldehyde concentration. The complex bioluminescence decay kinetics are interpreted in terms of a scheme, where bacterial luciferase is able t o perform multiple turnovers using different flavin species t o produce light. The initial phase of the bioluminescent reaction appears t o proceed mainly with fully reduced flavin as the substrate while the final one results from the involvement of flavin semiquinone in the catalytic cycle.