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Reversible dissociation of dimeric tyrosyl-tRNA synthetase by mutagenesis at the subunit interface

โœ Scribed by Jones, D. Hugh; McMillan, Andrew J.; Fersht, Alan R.; Winter, Greg

Book ID
126008739
Publisher
American Chemical Society
Year
1985
Tongue
English
Weight
667 KB
Volume
24
Category
Article
ISSN
0006-2960

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โœ Fersht, Alan R. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› American Chemical Society ๐ŸŒ English โš– 828 KB

To understand an enzyme reaction, one has to characterize the bound substrates, intermediates, products, and transition states on the reaction pathway and determine the interaction energies between them and the enzyme as the reaction proceeds. Site-directed mutagenesis is invaluable in this task, en