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Reversible cross-linking combined with immunoprecipitation to study RNA–protein interactions in vivo

✍ Scribed by Somashe Niranjanakumari; Erika Lasda; Robert Brazas; Mariano A. Garcia-Blanco


Book ID
117702057
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
332 KB
Volume
26
Category
Article
ISSN
1046-2023

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✍ Oste, Christian ;Brimacombe, Richard 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 578 KB

The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second