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Reversibility of the “irreversible” histidine ammonia-lyase reaction

✍ Scribed by Virginia R. Williams; Janice M. Hiroms

Book ID
115731434
Publisher
Elsevier Science
Year
1967
Weight
193 KB
Volume
139
Category
Article
ISSN
0005-2744

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On the nature of the irreversible inhibi
On the nature of the irreversible inhibition of histidine ammonia lyase by cysteine and dioxygen
✍ Karlheinz Weber; János Rétey 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 484 KB

The irreversible inhibition of histidine ammonia lyase by L-cysteine and dioxygen has been reexamined. After denaturation and consecutive digestion of the inhibited enzyme by trypsin and endoproteinase Glu-C, the generated chromophoric system (lambda max = 340 nm) remained intact and was isolated in