Jorgensen et al. ('54) showed that adult anurans may selectively be depleted of C1 independently of Na; after making C1 available to the animals the deficit was restored. In their study on the cutaneous uptake of C1 by intact frogs, Jorgensen et al. reported that some animals exhibited skin potenti
Reversed potentials in isolated frog skin. II. Active transport of chloride
β Scribed by Duncan W. Martin; Peter F. Curran
- Publisher
- John Wiley and Sons
- Year
- 1966
- Tongue
- English
- Weight
- 540 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0021-9541
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β¦ Synopsis
Net inward transport of C1 in the absence of an electrochemical potential difference was demonstrated in the skin of two species of frog, R. pipiens and R. esculenta under conditions of low ( 2 mM) C1 concentration in the bathing solutions. The electrical potential profle of skins of R. pipiens was examined with microelectrodes under conditions in which the inside solution was negative relative to the outside solution. This reversal of the normal potential difference was found to arise 2 s a result of potential changes across the outward facing electrical barriers in the skin. The reversed potential difference appears to arise, at least in part, as a result of the inward Cl transport. The effect may be due either to electrogenic C1 transfer or to variations in internal composition of the epithelial cells arising as a result of C1 transport.
π SIMILAR VOLUMES
Since the pioneer work of du Bois Reymond ( '57), the origin of the potential across the isolated frog skin, a i d the anatomical location of the active site, or sites, has bccn a subject of considera1)le debate arnong physiologists. The predominant, hut by no means unanimous, opinion has bccn that
## NINE FIGURES This paper deals with (1) the production of a hydrogen ion gradient across the isolated frog skin, (2) the relationship between the gradient and the active transport of sodium ion, and (3) the effect of potassium ion and aearation on the hydrogen ion gradient and the transport proc