𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Reversed-phase high-performance liquid chromatography of fatty acid methyl esters with particular reference to cyclopropenoic and cyclopropanoic acids

✍ Scribed by Bianchini, J.-P. ;Ralaimanarivo, A. ;Gaydou, E. M.

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
439 KB
Volume
5
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

High performance liquid chromatography of saturated, monounsaturated, diunsaturated, triunsaturated, cyclopropenoic (malvalic and sterculic) and cyclopropanoic (cis‐8,9‐methylenehexadecanoic and dihydrosterculic) fatty acids was performed with their methyl esters. All separations were carried out with two types of reversed phase columns, the eluent consisting of an acetonitrile/water mixture. The effect of water was studied in the range 0–15%. The best separation was obtained with acetonitrile/water (85:15 v/v). Quantitative results indicated that the detection limits depended upon ultraviolet wavelength and in the present study were 4 ng of methyl sterculate and 125 ng of methyl dihydrosterculate at 195 nm.

📜 SIMILAR VOLUMES

Reversed-phase high-performance thin-lay
Reversed-phase high-performance thin-layer chromatography of mono-, di- and trisubstituted bile acid methyl esters
✍ Iida, T. ;Yamauchi, C. ;Chang, F. C. 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 284 KB 👁 2 views

## Abstract A comparative study is reported on separation of series of mono‐, di‐, and trisubstituted methyl 5β‐cholanates, which differ only in the position and stereochemistry of hydroxyl or keto groups at position and stereochemistry of hydroxyl or keto groups at positions C‐3, C‐7, and/or C‐12,

High-Performance Liquid Chromatography o
High-Performance Liquid Chromatography of α-Amino acids and Aztreonam on Reversed Phase Columns with Aqueous Cu2+ as Eluent
✍ Alaa Khedr 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 364 KB

Parameters affecting the separation of amino acids on different RP-HPLC columns were studied. Six amino acids were separated on Zorbax TMS, Zorbax CN, Zorbax ODS and Zorbax C,, using 1.8 x lo-' M copper sulphate at PH 4.1 as aqueous mobile phase. The best separation was shown by Zorbax TMS followed