High-Performance Liquid Chromatography of α-Amino acids and Aztreonam on Reversed Phase Columns with Aqueous Cu2+ as Eluent

✍ Scribed by Alaa Khedr

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 364 KB
• Volume: 10
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199607)10:4<167::aid-bmc584>3.0.co;2-#

✦ Synopsis

Parameters affecting the separation of amino acids on different RP-HPLC columns were studied. Six amino acids were separated on Zorbax TMS, Zorbax CN, Zorbax ODS and Zorbax C,, using 1.8 x lo-' M copper sulphate at PH 4.1 as aqueous mobile phase. The best separation was shown by Zorbax TMS followed by Zorbax CN. The column performance was maintained by serial washing after 6 h continuous work with aqueous lo-' M disodium edehte, lo-' N sulphuric acid, water and gradient elution with aqueous methanol. The separation mechanism was interpreted. The method was applied for separation and quantification of aztreonam and L-arginine in Azactam vials.