## Analysis of camptothecins in biologic media is hampered by chemical hydrolysis of the parent lactone (form I) to an inactive hydroxy acid (form II). A solidphase extraction (SPE) method utilizing C2 -bonded silica particles (100 mg, 1 ml) is presented for simultaneous determination of forms I a
Reversed-phase high-performance liquid chromatographic method for the simultaneous quantitation of the carboxylate and lactone forms of the camptothecin derivative irinotecan, CPT-11, and its metabolite SN-38 in plasma
β Scribed by Laurent P. Rivory; Jacques Robert
- Publisher
- Elsevier Science
- Year
- 1994
- Weight
- 698 KB
- Volume
- 661
- Category
- Article
- ISSN
- 0378-4347
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## Abstract A simple and sensitive assay for glucuronidation activity of 7βethylβ10βhydroxycamptothecin (SNβ38), the active metabolite of irinotecan (CPTβ11), in human liver microsomes by highβperformance liquid chromatography (HPLC) with fluorescence detection is reported. The method was validated
## Acknowledgements The authors thank the Upjohn Co., Kalamazm, Mich.. for the generous supplies of tolbutamide. One of the authors (P. M.) gratefully acknowledges the financial support of the Delegation Generale Recherche Sciences et Techniques (Paris, France).
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