A nonfunctional immunoglobulin kappa-chain gene previously shown to contain intracisternal A-particle (IAP) sequences within one of its introns was further characterized by DNA and RNA blot analysis. The results of these experiments indicate that the joining of the IAP sequences to the kappa-chain g
Retrovirus particles of mouse hybridomas
โ Scribed by A.H. Bartal; R. Ludatscher; Ch. Lichtig; R. Erlandson; C. Feit; E. Robinson; Y. Hirshaut
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 98 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0304-3991
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Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell grow