Retrovirus-mediated herpes simplex virus thymidine kinase gene therapy approach for hepatocellular carcinoma
β Scribed by GAO, Ding Cheng; AN, Wei; DAI, Jie
- Book ID
- 110020387
- Publisher
- Nature Publishing Group
- Year
- 1999
- Tongue
- English
- Weight
- 447 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0007-0610
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## Background: The suicide gene and prodrug, herpes simplex thymidine kinase (hstk) and ganciclovir (gcv), are now in clinical trials for recurrent malignancies. ## Methods: We evaluated in vitro and in vivo efficacy of hstk gene transfer and gcv treatment of colonic adenocarcinoma in a syngeneic
We have analyzed the ability of a recombinant replication-defective adenovirus to transfer the thymidine kinase gene of herpes simplex virus (HSV-tk) into hepatocellular carcinoma (HCC) cells to confer sensitivity to ganciclovir. Three HCC cell lines (Hep3B, PLC/PRF/5, and HepG2) were efficiently in