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Retion of human apolipoprotein A-II by a mutant ofSaccharomyces cerevisiæ

✍ Scribed by M. Pascual; P. Richard; C. Hamon; M. Aigle; A. Iron

Book ID
104640013
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
482 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

A modified cDNA of mature human apolipoprotein A-II (apoA-II) was expressed by a sterol-uptake yeast strain (erglo) of Saccharomyces cerevisia?. ApoA-II cDNA wax fused with a modified yeast alpha factor leader peptide coding sequence under pbosphoglycerate lcinase promoter control in a 2 micron-based plasmid construction. The use of cholesterol allowed apoA-II secretion in the culture medium. The mono and dimeric forms of apoA-II (approximately 3 microgmms perml)weredetected

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