✦ LIBER ✦

Retention in capillary supercritical fluid chromatography

✍ Scribed by Chester, T. L. ;Innis, D. P.

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 501 KB
Volume: 8
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240080917

No coin nor oath required. For personal study only.

✦ Synopsis

The data so far published on this subject are not very satisfactory [S-lo]. Not only the results but also the dimensions used for expression of enzyme activities differ considerably and are confusing. 5a-reducing enzymes are commonly evaluated by measuring the metabolic conversion rates of a labeled precursor to distinct products followed by calculation of the reaction velocities based on the sum of all conversion rates.

We recommend measuring specific radioactivities of all peaks used for calculation of reaction velocities to avoid quantitative errors which may arise from dilution processes caused by endogenous hormone contents. This can occur especially in cases of patients suffering from disorders such as androgen insensitivity syndrome. Radio gas chromatography for qualitative and quantitative analysis of the incubation mixtures is a convenient way to overcome this disadvantage. Simultaneous detection of mass and radioactivity of the chromatogram peaks allows rapid calculation of specific radioactivity of all metabolites of interest.

References

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