Restriction fragment length polymorphism detected by human salivary amylase cDNA
β Scribed by Kanji Ishizaki; Asao Noda; Mituo Ikenaga; Kenji Ida; Keiichi Omoto; Yusuke Nakamura; Ken-ichi Matsubara
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 146 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
The plasmid clone which contains human salivary amylase cDNA was used to detect restriction fragment length polymorphisms (RFLPs). After double digestion with Pst 1 and Bam H1, a polymorphism with two alleles was observed. In Japanese, frequencies of these alleles, tentatively called 5.7 kb and 6.5 kb fragment alleles, are 0.55 and 0.45, respectively.
π SIMILAR VOLUMES
Three anonymous DNA probes ACH207, ACH224, and ACH202, isolated from a flow-purified chromosome 16 library and mapped to defined intervals of human chromosome 16, detected restriction fragment length polymorphisms (RFLPs). The RFLPs were of simple two allele types. The ACH207 (D16S4) probe detected
A simplified version of a PCR-based reductional restriction fragment length polymorphism (rRFLP) approach for typing of human papillomaviruses (HPVs) is described previously . It is achieved by the use of a biotinlabeled primer in PCR which, on restriction digestion and staining, is associated with