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Restriction enzyme analysis of the Plasmid ColIb DNA

✍ Scribed by Skorupska, Anna ;Buraczyńska, Monika ;Lorkiewicz, Zbigniew

Publisher
Springer
Year
1979
Tongue
English
Weight
306 KB
Volume
173
Category
Article
ISSN
0026-8925

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✦ Synopsis

Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated. The total molecular weight of the fragments generated by EcoRI was 61.42 Mdal and for HindIII fragments 62.79 Mdal.

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