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Restriction endonuclease analysis of chromosomal DNA fromListeria monocytogenesstrains

✍ Scribed by C. Casolari; B. Facinelli; U. Fabio; J. Rocourt; P. E. Varaldo

Publisher
Springer
Year
1990
Tongue
English
Weight
734 KB
Volume
6
Category
Article
ISSN
0393-2990

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✦ Synopsis

Restriction endonuclease analysis of chromosomal DNA was applied to thirteen Listeria monocytogenes strains alongside the more conventional typing methods of serotyping and phage typing. The organisms were isolated from cases of sporadic listeriosis (nine strains); from an occasional nosocomial cluster (two strains); and from food samples (two strains). Purified DNAs were digested with EcoRI restriction endonuclease, and restriction fragments separated by electrophoresis. Restriction patterns correlated well with phage patterns, but also allowed typing of the phage-untypable strains. DNA fingerprinting appears to be a potentially helpful tool for epidemiological investigations of listeric infections, particularly when phage typing fails to determine the identity or diversity of the isolates.

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