𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases

✍ Scribed by J. H. Meyerink; J. Retèl; R. J. Planta; F. Heidekamp

Publisher
Springer
Year
1976
Tongue
English
Weight
353 KB
Volume
2
Category
Article
ISSN
0301-4851

No coin nor oath required. For personal study only.

✦ Synopsis

Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous 'spacer' sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed.

📜 SIMILAR VOLUMES

Restriction endonuclease analysis of rib
Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae
✍ Cramer, Jane Harris ;Farrelly, Frances W. ;Rownd, Robert H. 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 874 KB

The size and degree of homogeneity of the repetitive units in purified ribosomal DNA (gamma DNA) from Saccharomyces cerevisiae have been analyzed by restriction endonuclease digestion and heteroduplex mapping. Digestion of the gamma DNA with EcoRI yields seven fragments, digestion with Hind II+III y